| Organisation | May 1861 | April 1862 | January 1863 |
| Colonel | George Maney | Hume R. Field | Hume R. Feild |
| Lieutenant-Colonel | T. F. Sevier | John Patterson | John L. House |
| Major | Abraham M. Looney | John L House | A. C. Allen |
| Adjutant | Robert B. Snowden | Thomas H. McKinney | Thomas H. McKinney |
| Surgeon | W. L. Nichol | J. R. Buist | J. R. Buist |
| Assistant Surgeon | J. R. Buist | W. R. Hughes | W. R. Hughes |
| A.Q.M. | Samuel H. Ransom | M. B. Pilcher | A. S. Camp |
| A.C.S. | George W. Menees | George W. Menees | |
| Co. A. Captain | Joseph Vaulx | William D Kelly | |
| Co. B. Captain | J. B. Craighead J. Patterson | Bailey P Steele | |
| Co. C. Captain | Robert C Foster | John F. Wheless | |
| Co. D. Captain | James P. Hanna | Oscar Adkinson | |
| Co. E. Captain | George Harsch | George Leascher | |
| Co. F. Captain | John L Butler | John L Butler | |
| Co. G. Captain | George L. U. Campbell | __ Irvine | |
| Co. H. Captain | William R Johnson | Harry Webster (soon replaced by Jo. P. Lee) | |
| Co. I. Captain | William Ledbetter | William Ledbetter | |
| Co. K. Captain | Hume R. Feild | William C. Flournoy | |
| Co. L. Captain | J. W. Fulcher | ||
| Consolidation of 1st/27th (January 1863): | |||
| Consolidated | Formerly | Captains | |
| Co. A | Co. I | William Ledbetter | |
| Co. B | 27th TN | Capt. Lawler | |
| Co. C | Co. K | William C. Flournoy | |
| Co. D | Co. L | J. W. Fulcher | |
| Co. E | Cos. A/B/C | William D Kelly | |
| Co. F | Co. D | Oscar Adkinson | |
| Co. G | Cos. E/F/G | Ben Smith | |
| Co. H | Co. H | Joseph P. Lee | |
| Co. I | 27th TN | Capt. Beasley | |
| Co. K | 27th TN | Capt. Perry | |